1. León-Vaz A, León R., Giráldez I., Vega JM., Vigara J. Impact of heavy metals in the microalga Chlorella sorokiniana and assessment of its potential use in cadmium bioremediation. Aquatic Toxicology239; 105941, 2021 (4.9, D1).

2. Valero Y, González-Fernández C, Cárdenas C, Guzmán F, León R, Cuesta. ANK-lysin peptides ameliorate viral encephalopathy and retinopathy disease and produce partial protection against nodavirus infection. Antiviral Research 2021, 532, 15, 2021. (4.1, Q1).

3.Constantino A; Rodrigues B., Leon R, Barros R, Raposo S.  Alternative chemo-enzymatic hydrolysis strategy applied to different microalgae species for bioethanol production. Algal Research. 56, 102329. 2021. (4.0, Q1)

4.Cuesta A., Vila M, Molina-Marquez M, Carbajo C, Lebron JA, López-López M, López-Cornejo P, Moyá ML, López-Sanmartín M, López JR, Navas JJ., Vigara J, León R. Influence of adding terminal tags on the structural and antimicrobial properties of the peptide caerin 1.1. Aquaculture. 532, 15, 2021. (3.22, D1)

5.León-Vaz A, Romero LC, Gotor C, León R, Vigara J.  Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study. Ecotoxicology and Environmental Safety 207 111301. 2021. (6.291, D1) DOI: 10.1016/j.ecoenv.2020.111301.

6. Gómez-Villegas, P.; Vigara, J.; Romero, L.; Gotor, C.; Raposo, S.; Gonçalves, B.; Léon, R. Biochemical Characterization of the Amylase Activity from the New Haloarchaeal Strain Haloarcula sp. HS Isolated in the Odiel Marshlands. Biology 2021, 10, 337.(3. 79, Q1)


1. Gómez‐Villegas P, J. Vigara, M. Vila, J. Varela, L. Barreira. and R. Léon. Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain). Biology 2020, 9, 298. (3. 79, Q1); doi:10.3390/biology9090298

2. León R, Ruiz M, Valero Y, Cárdenas C, Guzman F, Vila M, Cuesta A. Exploring small cationic peptides of different origin as potential antimicrobial agents in aquaculture. Fish Shellfish Immunol. 2020, 98: 720-727. IF: 3.30, Q1. DOI:10.1016/j.fsi.2019.11.019.

3. Kaouther Sahli, Mohamed A. Gomri, Julia Esclapez, Patricia Gomez‐Villegas, Oulaya Ghennai, Maria‐Jose Bonete, Rosa Leon, Karima Kharrou. Bioprospecting and characterization of pigmented halophilic archaeal strains from Algerian hypersaline environments with analysis of carotenoids produced by Halorubrum sp. BS2. J. Basic Microbiology. 2020. DOI: 10.1002/jobm.202000083

4. Lisa M. Schüler, Paulo Duarte, Chloé Placines, Ana María Molina Márquez, Rosa Léon-Bañares, Katkam N. Gangadhar, João Varela and Luísa Barreira. Improvement of carotenoid extraction from a recently isolated, robust microalga, Tetraselmis sp. CTP4 (Chlorophyta). Bioprocess and Biosystems Engineering. 2020, 1-12. DOI: https://doi:10.1007/s00449-019-02273-9

5. Molina-Márquez a, Vila M, Rengel R, Fernández E, Garcia-Maroto F, Vigara J, León R. Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae, 2020, Int. J. Mol. Sci. 21(3), 718. DOI:


1. León-Vaz A León R., Díaz-Santos E., Vigara J., Raposo S. Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana. New Biotechnology.51: 31-38 (2019).DOI:

2. Ana Molina-Márquez, Marta Vila, Javier Vigara, Ana Borrero and Rosa León. The Bacterial Phytoene Desaturase-Encoding Gene (CRTI) is an Efficient Selectable Marker for the Genetic Transformation of Eukaryotic Microalgae. Metabolites. 9, 49 (2019). DOI: doi:10.3390/metabo9030049


1. Patricia Gómez-Villegas, Javier Vigara and Rosa León. Characterization of the Microbial Population Inhabiting a Solar Saltern Pond of the Odiel Marshlands (SW Spain). Mar. Drugs. 2018, 16, 332. DOI:

2. Rengel R, Smith RT, Haslam R, Sayanova O, Vila M, León R. Overexpression of acetyl-CoA synthetase (ACS) enhances the biosynthesis of neutral lipids and starch in the green microalga Chlamydomonas reinhardtii. Algal Research. 31: 183-193 (2018).


1. Schüler LM., Peter S.C. Schulze, Hugo Pereira, Luísa Barreira, Rosa León and João Varela. Trends and strategies to enhance carotenoid, TAG and LC-PUFA production in microalgae. Algal Research. 25: 263–273 (2017) (3.99, Q1).


1. Valverde F., Romero-Campereo FJ., León R., García-Guerrero M. and Serrano A. New Challenges in microalgal biotechnology Eur. J. protistol. 55:91-105 (2016). (2.58, Q2).

2. Díaz-Santos E., Vila M., Vigara J.,León R A new approach to express transgenes in microalgae and its use to increase the flocculation ability of Chlamydomonas reinhardtii J. Appl. Phycol. 28:1611-1621 (2016). (2.56, Q1).

3. De la Vega M.; Sayago A., García-Barneto A.; Ariza J and León R. Isolation of a new bacterioruberin-producing haloarchaea from the marshlands of the Odiel river in the Southwest Spain Biotechnol. progr. 32(3):592-600 (2016). (2.15, Q1).


1. Varela, JC; Pereira H, Vila M, León R. Production of carotenoids by microalgae: achievements and challenges. Photosynth. Res. 125(3):423-36 (2015). (3.5, Q1).

2. Díaz-Santos E, Vila M, de la Vega M, León R, Vigara J. Study of bioflocculation Induced by Saccharomyces bayanus var. uvarum and flocculating protein factors in microalgae. Algae Research. 8: 23–29 (2015). (4.01, Q1).


1. Díaz-Santos E., Vega M.,Vila M., Vigara J., León R. Efficiency of different heterologous promoters in the microalga C. reinhardtii. Biotechnol. Prog. 29(2):319-28 (2013).(2.34, Q1).


1. Vila M., Díaz E., de la Vega M., Rodríguez H., Vargas A., León R. Promoter trapping in microalgae by inverse PCR using paromomycin as selective agent. Marine Drugs 10: 2749-2765 (2012). (3.85, Q1).


1. M de la Vega; E. Díaz, M Vila and R León. Isolation of a new strain of Picochlorum sp and characterization of its potential biotechnological applications. Biotechnol. Prog. 27(6):1535-43 (2011). (2.34, Q1).

2. Cordero BF, Couso I, León R, Rodríguez H, Vargas MA. Enhancement of carotenoids biosynthesis in C. reinhardtii by nuclear transformation using a phytoene synthase gene isolated from C. zofingiensis. Appl. Microb. Biotechnol. 91(2):341-51 (2011). (3.43, Q1).

3. Couso, M Vila , H Rodríguez, M Vargas and R León. Overexpression of an exogenous phytoene synthase gene in the unicellular alga Chlamydomonas reinhardtii leads to an increase in the content of carotenoids. Biotechnol. Prog. 27(1):54-60 ( 2011). (2.34, Q1).


1. BF Cordero, I Obraztsova, L Martín, I Couso, R León, MA Vargas and H Rodríguez. Isolation and characterization of a lycopene β-cyclase gene from Chlorella zofingiensis. J. Phycol. 46(6): 1229-1236 (2010). (2.24, Q1).


1. M. Vila, I. Couso and R. León. Carotenoid content in mutants of the chlorophyte Chlamydomonas reinhardtii with low expression levels of phytoene desaturase. Process Biochem. 42:1147-1152 (2008). (2.4, Q1).