{"id":4926,"date":"2024-02-21T13:47:40","date_gmt":"2024-02-21T12:47:40","guid":{"rendered":"https:\/\/www.uhu.es\/rensma\/?page_id=4926"},"modified":"2026-03-02T12:24:55","modified_gmt":"2026-03-02T11:24:55","slug":"publications-mgof","status":"publish","type":"page","link":"https:\/\/www.uhu.es\/rensma\/en\/publications-mgof\/","title":{"rendered":"Publications Genetic Engineering Of Photosynthetic Organisms Unit"},"content":{"rendered":"<div id=\"gutenslider-ntlgwil82\" class=\"wp-block-eedee-block-gutenslider gs-frontend content-change alignfull arrow-style-1 arrow-position-sides arrow-mb-none arrows-lg arrows-md arrows-sm dot-style-1 dots-lg dots-md dots-sm dot-position-bottom is-position-center-center mb-none wrap-auto-height\" data-parallax-direction=\"down\" data-parallax-amount=\"1.3\" data-lg-transition=\"lg-slide\" data-hash=\"\" ><div class=\"eedee-swiper-outer\" style=\"--gutenslider-min-height: 430px;--gutenslider-arrow-size: 30px;--gutenslider-dot-size: 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has-larger-font-size\"><\/p>\n\n<\/div><\/div>\n\n<div class=\"swiper-slide wp-block-eedee-block-gutenslide is-position-center-center ed-bg-image\" style=\"--ed-vw:300;--ed-vh:99;\" data-hash=\"\"><div class=\"eedee-background-div bg-image\"><img loading=\"lazy\" width=\"1024\" height=\"337\" class=\"wp-image-3110\" src=\"https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/4-1024x337.jpg\" alt=\"\" decoding=\"async\" style=\"object-fit: cover; object-position: 50% 50%;\" srcset=\"https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/4-1024x337.jpg 1024w, https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/4-300x99.jpg 300w, https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/4-768x253.jpg 768w, https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/4.jpg 1032w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><div class=\"eedee-background-div__overlay\" style=\"opacity:1;\"><\/div><\/div><div class=\"slide-content\">\n\n<p class=\"has-text-align-center gutenslider-content-initial has-larger-font-size\"><\/p>\n\n<\/div><\/div>\n\n<div class=\"swiper-slide wp-block-eedee-block-gutenslide is-position-center-center ed-bg-image\" style=\"--ed-vw:300;--ed-vh:99;\" data-hash=\"\"><div class=\"eedee-background-div bg-image\"><img loading=\"lazy\" width=\"1024\" height=\"337\" class=\"wp-image-3112\" src=\"https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/6-1024x337.jpg\" alt=\"\" decoding=\"async\" style=\"object-fit: cover; object-position: 50% 50%;\" srcset=\"https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/6-1024x337.jpg 1024w, https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/6-300x99.jpg 300w, https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/6-768x253.jpg 768w, https:\/\/www.uhu.es\/rensma\/wp-content\/uploads\/2021\/01\/6.jpg 1032w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><div class=\"eedee-background-div__overlay\" style=\"opacity:1;\"><\/div><\/div><div class=\"slide-content\">\n\n<p class=\"has-text-align-center gutenslider-content-initial has-larger-font-size\"><\/p>\n\n<\/div><\/div>\n<\/div><\/div><button class=\"eedee-gutenslider-nav eedee-gutenslider-prev\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 24 24\" width=\"24\" height=\"24\"><circle class=\"bg\" cx=\"13\" cy=\"12\" r=\"11\" \/><path fill=\"none\" d=\"M0 0h24v24H0z\"\/><path class=\"arrow\" d=\"M10.828 12l4.95 4.95-1.414 1.414L8 12l6.364-6.364 1.414 1.414z\"\/><\/svg><\/button><button class=\"eedee-gutenslider-nav eedee-gutenslider-next\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 24 24\" width=\"24\" height=\"24\"><circle class=\"bg\" cx=\"11\" cy=\"12\" r=\"11\" \/><path fill=\"none\" d=\"M0 0h24v24H0z\"\/><path class=\"arrow\" d=\"M13.172 12l-4.95-4.95 1.414-1.414L16 12l-6.364 6.364-1.414-1.414z\"\/><\/svg><\/button><div class=\"eedee-gutenslider-pagination\"><\/div><div class=\"gutenslider-divider-bottom \" style=\"color:#fff;\"><\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h1 class=\"wp-block-heading has-text-align-center\">Publications<\/h1>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\" style=\"text-transform:uppercase\"><strong>Genetic Engineering Of Photosynthetic Organisms Unit<\/strong><\/h3>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-5 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<p class=\"has-ast-global-color-5-color has-ast-global-color-0-background-color has-text-color has-background\">Laboratory Menu<\/p>\n\n\n<nav class=\" is-vertical wp-block-navigation is-layout-flex wp-container-2 wp-block-navigation-is-layout-flex\" aria-label=\"Menu MGM HOLA\"><ul class=\"wp-block-navigation__container  is-vertical wp-block-navigation\"><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/introduction-mgof\/\"><span class=\"wp-block-navigation-item__label\">Presentation<\/span><\/a><\/li><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/research-lines-mgof\/\"><span class=\"wp-block-navigation-item__label\">Research Lines<\/span><\/a><\/li><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/members-mgof\/\"><span class=\"wp-block-navigation-item__label\">Members<\/span><\/a><\/li><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/projects-mgof\/\"><span class=\"wp-block-navigation-item__label\">Projects<\/span><\/a><\/li><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/publications-mgof\/\"><span class=\"wp-block-navigation-item__label\">Publications<\/span><\/a><\/li><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/phd-theses-mgof\/\"><span class=\"wp-block-navigation-item__label\">PhD Theses<\/span><\/a><\/li><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/infrastructures-mgof\/\"><span class=\"wp-block-navigation-item__label\">Infraestructures<\/span><\/a><\/li><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/collaborations-mgof\/\"><span class=\"wp-block-navigation-item__label\">Collaborations<\/span><\/a><\/li><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  href=\"https:\/\/www.uhu.es\/rensma\/en\/\"><span class=\"wp-block-navigation-item__label\">\u226a Return \u2026<\/span><\/a><\/li><\/ul><\/nav><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h2 class=\"wp-block-heading\">INTERNATIONAL PUBLICATIONS<\/h2>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>202<\/strong>6<\/h3>\n\n\n\n<p><strong>Molina\u2010M\u00e1rquez, A, S. Kelterborn, P. Hegemann, M. P\u00e9rezRodr\u00edguez, J. Vigara and R. Le\u00f3n<\/strong>. 2026. Characterization of Phytoene Desaturase Knockout Carotenoid\u2010Deficient Microalgal Mutants Generated by Cas9\u2010Ribonucleoprotein Complexes. Physiologia Plantarum e70811.\u00a0<a rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.1111\/ppl.70811\" target=\"_blank\">https:\/\/doi.org\/10.1111\/ppl.70811<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>202<\/strong>5<\/h3>\n\n\n\n<p><strong>\u00c1ngeles Mor\u00f3n-Ortiz, Mar Ferrando-Marco, Antonio Le\u00f3n-Vaz, Rosa Le\u00f3n, Paula Mapelli-Brahm, Michalis Barkoulas, Mel\u00e9ndez Mart\u00ednez, Antonio Jes\u00fas,<\/strong> Effects of Lutein, Phytoene and Carotenoid-rich Microalgal Extracts on the epidermis of Caenorhabditis elegans. FOODCHEM-&nbsp;&nbsp;<a href=\"callto:497, 30 2025, 147022\">497, 30 2025, 147022<\/a><\/p>\n\n\n\n<p><strong>Mor\u00f3n-Ortiz, \u00c1ngeles\u1d43; Ben\u00edtez Gonz\u00e1lez, Ana M.\u1d43, Le\u00f3n-Vaz, Antoniob; Le\u00f3n, Rosab, Mapelli-Brahm, Paula\u1d43*; Mel\u00e9ndez Mart\u00ednez, Antonio Jes\u00fas<\/strong>. Effect of ultrasounds and ball milling on the bioaccessibility of carotenoids from the microalga Dunaliella bardawil&nbsp; 2025<\/p>\n\n\n\n<p><strong>Carolina Gallardo Ybarra; Arturo S\u00e1nchez Paz; Jos\u00e9 Manuel Grijalva Chon; Christian Minjarez Osorio; Rosa Le\u00f3n Ba\u00f1ares; Ana Molina M\u00e1rquez; Chrystian Mariana Rodr\u00edguez Armenta; Carla Mar\u00eda Badilla Valenzuela; Enrique De la Re V<\/strong>. Transformaci\u00f3n gen\u00e9tica de Chlamydomonas reinharditti con un shARN asociado a un vector adenoviral dirigido al Ostreavirus ostreidmalaco Biotecnia. 2025-11-20 | Journal article. DOI: 10.18633\/biotecnia.v27.2767.&nbsp;&nbsp;<\/p>\n\n\n\n<p><strong>Tzlil G, Mar\u00edn MDC, Matsuzaki Y, Nag P, Itakura S, Mizuno Y, Murakoshi S, Tanaka T, Larom S, Konno M, Abe-Yoshizumi R, Molina-M\u00e1rquez A, B\u00e1rcenas-P\u00e9rez D, Cheel J, Kobl\u00ed\u017eek M, Le\u00f3n R, Katayama K, Kandori H, Schapiro I, Shihoya W, Nureki O, Inoue K, Rozenberg A, Chazan A, B\u00e9j\u00e0 O. <\/strong>Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea. Nat Microbiol. 2025 Jun;10(6):1484-1500. doi: 10.1038\/s41564-025-02016-5. Epub 2025 May 29. PMID: 40442502; PMCID: PMC12137139.Principio del formulario<\/p>\n\n\n\n<p><strong>Morales-Salmer\u00f3n L, Fern\u00e1ndez-Boy E, Le\u00f3n R, Navarro-Fern\u00e1ndez CM, Dom\u00ednguez MT.<\/strong> Influence of the quality of organic amendments on Mediterranean agricultural soils following a drought episode. J Environ Manage. 2025 Apr;380:125093. doi: 10.1016\/j.jenvman.2025.125093. Epub <strong>2025<\/strong> Mar 25. PMID: 40138931.<\/p>\n\n\n\n<p><strong>Ishita Das, Ariel Chazan, Jonathan R. Church, Shirley Larom, Rosa Le\u00f3n, Patricia G\u00f3mez-Villegas, Daniela B\u00e1rcenas-P\u00e9rez, Jos\u00e9 Cheel, Michal Kobl\u00ed\u017eek, Oded B\u00e9j\u00e0, Igor Schapiro, and Mordechai Sheves.<\/strong> Selective Choice of the Efficient Carotenoid Antenna by a Xanthorhodopsin: Controlling Factors for Binding and Excitation Energy Transfer. <em>JACS Au<\/em>&nbsp; 2025 Article ASAP<strong>. <\/strong>DOI: 10.1021\/jacsau.4c01243<\/p>\n\n\n\n<p><strong>Morales-Salmer\u00f3n, L., Fern\u00e1ndez-Boy, E., Herrador, B. et al.<\/strong> Does an enhanced microbial diversity promote the resistance of soil multifunctionality against drought events in amended soils?. Biol Fertil Soils 61, 1013\u20131031 (2025). https:\/\/doi.org\/10.1007\/s00374-025-01914-4<\/p>\n\n\n\n<p><strong>Ana Molina-M\u00e1rquez, Manuel L\u00f3pez-L\u00f3pez, Roc\u00edo Rengel, Monserrat L\u00f3pez-Sanmart\u00edn, Elena Dom\u00ednguez, Roc\u00edo Pereira-Jaramillo, Pilar L\u00f3pez-Cornejo, Jos\u00e9 Antonio Lebr\u00f3n, Gloria Perazzoli, Jos\u00e9 Carbajo-Cobacho, Rosa Le\u00f3n. <\/strong>Characterization of PLGA nanoparticles for stabilization and controlled release of antimicrobial peptides into marine bivalves. Aquaculture,613,2026,743334 https:\/\/doi.org\/10.1016\/j.aquaculture.2025.743334.<\/p>\n\n\n\n<p><strong>Rastrojo-Velasco, J.M.; Leon, R.; Sayago, A.; Fernandez-Recamales, A.; Vigara, J.; Leon-Vaz, A.<\/strong> Effect Produced by a Mixture of Phenol, p-Cresol, and Acetophenone on Four Species of Microalgae: Tolerance, Biodegradation, and Metabolic Alterations. Toxics 2025, 13, 848. <a href=\"https:\/\/doi.org\/10.3390\/toxics13100848\">https:\/\/doi.org\/10.3390\/toxics13100848<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>202<\/strong>4<\/h3>\n\n\n\n<p><strong>Luque, C.; Perazzoli, G.; G\u00f3mez-Villegas, P.; Vigara, J.; Mart\u00ednez, R.; Garc\u00eda-Beltr\u00e1n, A.; Porres, J.M.; Prados, J.; Le\u00f3n, R.; Melguizo, C.<\/strong> Extracts from Microalgae and Archaea from the Andalusian Coast: A Potential Source of Antiproliferative, Antioxidant, and Preventive Compounds. J. Mar. Sci. Eng. <strong>2024<\/strong>, 12, 996. https:\/\/doi.org\/10.3390\/jmse12060996<\/p>\n\n\n\n<p><strong>Mar\u00eda del Carmen Romero-Cruz, Antonio Leon-Vaz, Inmaculada Gir\u00e1ldez, Jos\u00e9 Mar\u00eda Vega, Javier Vigara. <\/strong>Effect of heavy metals on the antioxidant system of the acid-tolerant microalga Coccomyxa onubensis. Algal Research. Vol, 77. 2024. DOI:10.1016\/j.algal.2023.103337<\/p>\n\n\n\n<p><strong>\u00c1ngeles Mor\u00f3n-Ortiz, Paula Mapelli-Brahm, Antonio Le\u00f3n-Vaz, Ana M. Benitez-Gonz\u00e1lez, Rosa Le\u00f3n, Antonio J. Mel\u00e9ndez-Mart\u00ednez. <\/strong>Ultrasound-assisted extraction of carotenoids from phytoene-accumulating Chlorella sorokiniana microalgae: Eff ect of milling and performance of the green biosolvents 2-methyltetrahydrofuran and ethyl lactate. Food Chem. 2024, 434,137437. DOI: 10.1016\/j.foodchem.2023.137437<\/p>\n\n\n\n<p><strong>Rosario Mart\u00ednez, Alejandro Garc\u00eda Beltr\u00e1n, Garyfallia Kapravelou, Ana Guzm\u00e1n, A\u00edda Lozano, Patricia G\u00f3mez-Villegas, Rosa Le\u00f3n, Javier Vigara, Milagros Galisteo, Pilar Aranda, Mar\u00eda L\u00f3pez-Jurado, Jos\u00e9 Prados, Consolaci\u00f3n Melguizo, Jesus M. Porres. <\/strong>Nutritional and functional assessment of haloarchaea and microalgae from the Andalusian shoreline: Promising functional foods with a high nutritional value. Func. Foods 116, 2024,106194, 1756-4646 https:\/\/doi.org\/10.1016\/j.jff .2024.106194.<\/p>\n\n\n\n<p><strong>Cristina Luque, Gloria Perazzoli, Patricia G\u00f3mez-Villegas, Javier Vigara, Rosario Mart\u00ednez, Alejandro Garc\u00eda-Beltr\u00e1n, Jes\u00fas M. Porres, Jose Prados, Rosa Le\u00f3n and Consolaci\u00f3n Melguizo. <\/strong>Extracts from Microalgae and Archaea from the Andalusian Coast: A Potential Source of Antiproliferative, Antioxidant, and Preventive Compounds. Mar. Sci. Eng. 2024, 12, 996. <a href=\"https:\/\/doi.org\/10.3390\/jmse12060996\">https:\/\/doi.org\/10.3390\/jmse12060996<\/a><\/p>\n\n\n\n<p><strong>Patricia G\u00f3mez-Villegas, Miguel P\u00e9rez-Rodr\u00edguez, Jes\u00fas M. Porres, Jos\u00e9 C. Prados, Consolaci\u00f3n Melguizo, Javier Vigara, Ignacio Moreno-Garrido, Rosa Le\u00f3n. <\/strong>Metataxonomy and pigments analyses unravel microbial diversity and the relevance of retinal-based photoheterotrophy at diff erent salinities in the Odiel Salterns (SW, Spain). Photochem. Photobiol. 2024. https:\/\/doi.org\/10.1016\/j.jphotobiol.2024.113043<\/p>\n\n\n\n<p><strong>Gloria Perazzoli, Cristina Luque, Antonio Le\u00f3n-Vaz, Patricia G\u00f3mez-Villegas, Roc\u00edo Rengel, Ana Molina-M\u00e1rquez, \u00c1ngeles Mor\u00f3n-Ortiz, Paula Mapelli-Brahm, Jos\u00e9 Prados, Consolaci\u00f3n Melguizo, Antonio Mel\u00e9ndez-Mart\u00ednez and Rosa Le\u00f3n. <\/strong>Preliminary Assessment of the Protective and Antitumor Eff ects of Several Phytoene-Containing Bacterial and Microalgal Extracts in Colorectal Cancer. Molecules 2024, 29, 5003 https:\/\/doi.org\/10.3390\/molecules29215003<\/p>\n\n\n\n<p><strong>\u00c1ngeles Mor\u00f3n-Ortiz, Paula Mapelli-Brahm, Antonio Le\u00f3n-Vaz, Ana M. Benitez-Gonz\u00e1lez, Andr\u00e9s N. Mart\u00edn-G\u00f3mez, Rosa Le\u00f3n, Antonio J. Mel\u00e9ndez-Mart\u00ednez. <\/strong>Assessment of milling and the green biosolvents ethyl lactate and 2-methyltetrahydrofuran (2-methyloxolane) for the ultrasound-assisted extraction of carotenoids in common and phytoene-rich Dunaliella bardawil microalgae. Food Sci. Techn. LWT, 213, 2024. 117007 https:\/\/doi.org\/10.1016\/j.lwt.2024.117007<\/p>\n\n\n\n<p><strong>Romero-Cruz MC, Le\u00f3n-Vaz A, Vega JM, Vigara J 2024 \u201cAlterations in nitrogen metabolism caused by heavy metals in the acid-tolerant microalga Coccomyxa onubensis\u201d <\/strong>Algal Research 84, 103784 DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.algal.2024.103784\">https:\/\/doi.org\/10.1016\/j.algal.2024.103784<\/a>.<\/p>\n\n\n\n<p><strong>Gali Tzlil, Mar\u00eda del Carmen Mar\u00edn, Yuma Matsuzaki, Probal Nag, Shota Itakura, Yosuke Mizuno, Shunya Murakoshi, Tatsuki Tanaka, Shirley Larom, Masae Konno, Rei Abe-Yoshizumi, Ana Molina-M\u00e1rquez, Daniela B\u00e1rcenas-P\u00e9rez, Jos\u00e9 Cheel, Michal Kobl\u00ed\u017eek, Rosa Le\u00f3n, Kota Katayama, Hideki Kandori, Igor Schapiro, Wataru Shihoya*, Osamu Nureki *, Keiichi Inoue*, Andrey Rozenberg*, Ariel Chazan* and Oded B\u00e9j\u00e01. Light-harvesting by antenna-containing rhodopsins in pelagic Asgard archaea. <\/strong>Nature Microbiol. In evaluation. bioRxiv, Sep 2024. DOI: 10.1101\/2024.09.18.613612<\/p>\n\n\n\n<p><strong>P\u00e9rez, D., Moy\u00e1, M.L., Bautista, M., Le\u00f3n, Rosa; Molina-M\u00e1rquez, Ana; Vila, Marta; Romero-Azogil, Luc\u00eda; Benito, Elena; de Gracia Garc\u00eda-Mart\u00edn, Mar\u00eda; Moreno-Gordillo, Paula; Rosado, Iv\u00e1n; Balestra, Fernando R.e. <\/strong>A novel biocompatible polymer derived from D-mannitol used as a vector in the fi eld of genetic engineering of eukaryotic cells. Colloids and Surfaces B: Biointerfaces, 2023, 224, 113219. Abril 2023 DOI: 10.1016\/j.colsurfb.2023.113219.<\/p>\n\n\n\n<p><strong>Chazan A, Das I, Fujiwara T, Murakoshi S, Rozenberg A, Molina-M\u00e1rquez A, Sano FK, Tanaka T, G\u00f3mez-Villegas P, Larom S, Pushkarev A, Malakar P, Hasegawa M, Tsukamoto Y, Ishizuka T, Konno M, Nagata T, Mizuno Y, Katayama K, Abe-Yoshizumi R, Ruhman S, Inoue K, Kandori H, Le\u00f3n R, Shihoya W, Yoshizawa S, Sheves M, Nureki O, B\u00e9j\u00e0 O. <\/strong>Phototrophy by antenna-containing rhodopsin pumps in aquatic environments. Nature. 2023 Mar; 615(7952):535-540. DOI: 10.1038\/s41586-023-05774-6.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2023<\/strong><\/h3>\n\n\n\n<p><strong>1.<\/strong> Anne Rolton, Stephen Charles Webb, Monserrat L\u00f3pez-Sanmart\u00edn, Kate Suzanne Hutson. Bivalve digestive epithelial virosis (DEV): A cause of disease or a natural process?. Journal of Invertebrate Pathology 198 (2023) 107924. <a href=\"https:\/\/doi.org\/10.1016\/j.jip.2023.107924\">https:\/\/doi.org\/10.1016\/j.jip.2023.107924<\/a> <\/p>\n\n\n\n<p><strong>2.<\/strong> Antonio Le\u00f3n-Vaz, Inmaculada Gir\u00e1ldez, Ignacio Moreno-Garrido, Joao Varela, Javier Vigara, Rosa Le\u00f3n, Jos\u00e9 Pedro Ca\u00f1avate. Amino acids profile of 56 species of microalgae reveals that free amino acids allow to distinguish between phylogenetic groups. Algal Research 74 (2023) 103181. <a href=\"https:\/\/doi.org\/10.1016\/j.algal.2023.103181\">https:\/\/doi.org\/10.1016\/j.algal.2023.103181<\/a><\/p>\n\n\n\n<p><strong>3.<\/strong> Antonio Le\u00f3n-Vaz, Rosa Le\u00f3n, Javier Vigara, Christiane Funk. Exploring Nordic microalgae as a potential novel source of antioxidant and bioactive compounds. New Biotechnology. 73 (25) 2023, 1-8. <a href=\"https:\/\/doi.org\/10.1016\/j.nbt.2022.12.001\">https:\/\/doi.org\/10.1016\/j.nbt.2022.12.001<\/a><\/p>\n\n\n\n<p>4<strong>.<\/strong> Antonio Leon-Vaz, Juan Cubero-Cardoso, Angeles Trujillo-Reyes C, Fernando G. Fermoso, Rosa Le\u00f3n, Christiane Funk, Javier Vigara, Juan Urbano. Enhanced wastewater bioremediation by a sulfur-based copolymer as scaffold for microalgae immobilization (AlgaPol). Chemosphere 315 (2023) 137761. <a href=\"https:\/\/doi.org\/10.1016\/j.chemosphere.2023.137761\">https:\/\/doi.org\/10.1016\/j.chemosphere.2023.137761<\/a><\/p>\n\n\n\n<p><strong>5.<\/strong> Monserrat L\u00f3pez-Sanmart\u00edn, Roc\u00edo Rengel, Manuel L\u00f3pez-L\u00f3pez, Antonio J. Lebr\u00f3n, Ana Molina-M\u00e1rquez, Ignacio de la Rosa, Pilar L\u00f3pez-Cornejo, Alberto Cuesta, Javier Vigara, Rosa Le\u00f3n. D-Amino acid peptides as antimicrobial agents against vibrio-associated diseases I aquaculture. Aquaculture. Volume 569, 2023, 739362. <a href=\"https:\/\/doi.org\/10.1016\/j.aquaculture.2023.739362\">https:\/\/doi.org\/10.1016\/j.aquaculture.2023.739362<\/a><\/p>\n\n\n\n<p><strong>6.<\/strong> Chazan A, Das I, Fujiwara T, Murakoshi S, Rozenberg A, Molina-M\u00e1rquez A, K. San F.K., Tatsuki Tanaka T, G\u00f3mez-Villegas P, Larom S, Pushkarev A, Malakar P, Hasegawa M, Tsukamoto Y, Ishizuka T, Konno M, Nagata T, Mizuno Y, Katayama K, Abe-Yoshizumi R, Ruhman S, Inoue K, Kandori H, Le\u00f3n R., Shihoya W, Yoshizawa S, Sheves M, Nureki O, and B\u00e9j\u00e0 O. Energy transfer in ubiquitous rhodopsin pumps with xanthophyll antennas. Nature. 2023 (In press). (69.5, D1). <a href=\"https:\/\/doi.org\/10.1038\/s41586-023-05774-6\">https:\/\/doi.org\/10.1038\/s41586-023-05774-6<\/a>. URL: <a href=\"https:\/\/www.nature.com\/articles\/s41586-023-05774-6\">https:\/\/www.nature.com\/articles\/s41586-023-05774-6<\/a><\/p>\n\n\n\n<p><strong>7.<\/strong> David P\u00e9rez a, Mar\u00eda Luisa Moy\u00e1 a, Mar\u00eda Bautista b, Rosa Le\u00f3n d, Ana Molina-M\u00e1rquez d, Marta Vila d, Luc\u00eda Romero-Azogil c, Elena Benito c, Mar\u00eda de Gracia Garc\u00eda-Mart\u00edn c, Paula Moreno-Gordillo f, Iv\u00e1n V. Rosado f, Fernando R. Balestra e, Pablo Huertas e, Manuel L\u00f3pez-L\u00f3pez b, Pilar L\u00f3pez-Cornejo. A novel biocompatible polymer derived from D-Mannitol used as a vector in the field of genetic engineering of Eukarytic cells. Colloids Surf. B., 224, 113219(1)-3219(9). <a href=\"https:\/\/doi.org\/10.1016\/j.colsurfb.2023.113219\">https:\/\/doi.org\/10.1016\/j.colsurfb.2023.113219<\/a><\/p>\n\n\n\n<p><strong>8.<\/strong> Javier \u00c1vila-Rom\u00e1n. Patricia G\u00f3mez-Villegas, Carla C. C. R. de Carvalho, Javier Vigara. Virginia Motilva. Rosa Le\u00f3n, Elena Talero Up-Regulation of the Nrf2\/HO-1 Antioxidant Pathway in Macrophages by an Extract from a New Halophilic Archaea Isolated in Odiel Saltworks. Antioxidants 2023, 12(5), 1080;<a href=\"https:\/\/doi.org\/10.3390\/antiox12051080\"> https:\/\/doi.org\/10.3390\/antiox12051080<\/a>&nbsp;<\/p>\n\n\n\n<p><strong>9.<\/strong> Paula Mapelli-Brahm, Patricia G\u00f3mez-Villegas, Mariana Lourdes Gonda, Antonio Le\u00f3n-Vaz, Rosa Le\u00f3n, Jennifer Mildenberger, C\u00e9line Rebours,Ver\u00f3nica Saravia, Silvana Vero,Eugenia Vila and Antonio J. Mel\u00e9ndez-Mart\u00ednez. Microalgae, Seaweeds and Aquatic Bacteria, Archaea, and Yeasts: Sources of Carotenoids with Potential Antioxidant and Anti-Inflammatory Health-Promoting Actions in the Sustainability Era. Mar. Drugs 2023, 21(6), 340; <a href=\"https:\/\/doi.org\/10.3390\/md21060340\">https:\/\/doi.org\/10.3390\/md21060340<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2022<\/strong><\/h3>\n\n\n\n<p><strong>1.<\/strong> Patricia G\u00f3mez-Villegas, Jos\u00e9 Luis Guerrero, Miguel P\u00e9rez-Rodriguez, Juan Pedro Bol\u00edvar, Antonio Morillo, Javier Vigara, Rosa L\u00e9on. Exploring the microbial community inhabiting the phosphogypsum stacksof Huelva (SW, SPAIN) by a high throughput 16S\/18S rDNA sequencing approach. Aquatic Toxicology. 2022. <a href=\"https:\/\/doi.org\/10.1016\/j.aquatox.2022.106103\">https:\/\/doi.org\/10.1016\/j.aquatox.2022.106103<\/a><\/p>\n\n\n\n<p><strong>2.<\/strong> Kaouther Sahli, Mohamed Amine Gomri, Julia Esclapez, G\u00f3mez-Villegas P., Bonete MJ, Le\u00f3n R and Kharroub K. Characterization and biological activities of carotenoids produced by three haloarchaeal strains isolated from Algerian salt lakes. Archives of Microbiology (2022) 204:6. <a href=\"https:\/\/doi.org\/10.1007\/s00203-021-02611-0\">https:\/\/doi.org\/10.1007\/s00203-021-02611-0<\/a><\/p>\n\n\n\n<p><strong>3.<\/strong> Juan Cubero-Cardoso, Patricia G\u00f3mez\u2010Villegas Mar\u00eda Santos-Mart\u00edn Ana Sayago, \u00c1ngeles Fern\u00e1ndez-Recamales, Rub\u00e9n Fern\u00e1ndez de Villar\u00e1n A. A. Cuadri Jos\u00e9 Enrique Mart\u00edn-Alfonso Rafael Borja Fernando Gonz\u00e1lez Fermoso Rosa Le\u00f3n Juan Urbano Combining vegetable oils and bioactive compounds via inverse vulcanization for antioxidant and antimicrobial materials. Polymer Testing 2022. Volume 109, May 2022, 107546<\/p>\n\n\n\n<p><strong>4.<\/strong> Patricia G\u00f3mez-Villegas, Jos\u00e9 Luis Guerrero, Miguel P\u00e9rez-Rodr\u00edguez, Juan Pedro Bolivar, Javier Vigara, Rosa Le\u00f3n. Metabarcoding data of prokaryotes and eukaryotes inhabiting the phosphogypsum stockpiles of the Odiel Marshlands in Huelva (S W, SPAIN). Data in brief. 41. 107989, 2022. <a href=\"https:\/\/doi.org\/10.1016\/j.dib.2022.107989\">https:\/\/doi.org\/10.1016\/j.dib.2022.107989<\/a><\/p>\n\n\n\n<p><strong>5.<\/strong> Rengel Roc\u00edo, Inmaculada Giraldez, Manuel J. D\u00edaz, Trinidad Garc\u00eda, Javier Vigara, Rosa L\u00e9on,*. Simultaneous production of carotenoids and chemical building blocks precursors from chlorophyta microalgae. Bioresource Technol. 2022 <a href=\"https:\/\/doi.org\/10.1016\/j.biortech.2022.127035\">https:\/\/doi.org\/10.1016\/j.biortech.2022.127035<\/a><\/p>\n\n\n\n<p><strong>6.<\/strong> Umaima Al Hoqani, Rosa Leon, Saul Purton: Over-expression of a cyanobacterial gene for 1-deoxy-D-xylulose-5-phosphate synthase in the chloroplast of Chlamydomonas reinhardtii perturbs chlorophyll:carotenoid ratios. Journal of King Saud University \u2013 Science 2022. <a href=\"https:\/\/doi.org\/10.1016\/j.jksus.2022.102141\">https:\/\/doi.org\/10.1016\/j.jksus.2022.102141<\/a><\/p>\n\n\n\n<p><strong>7.<\/strong> Sanjeet Mehariya, Martin Pl\u00f6hn, Antonio Le\u00f3n-Vaz, Alok Patel, Christiane Funk. Improving the content of high value compounds in Nordic Desmodesmus microalgal strains. Bioresource Technology, 359, 127455. (2022) <a href=\"https:\/\/doi.org\/10.1016\/j.biortech.2022.127445\">https:\/\/doi.org\/10.1016\/j.biortech.2022.127445<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2021<\/strong><\/h3>\n\n\n\n<p><strong>1.<\/strong> Le\u00f3n-Vaz A, Le\u00f3n R., Gir\u00e1ldez I., Vega JM., Vigara J. Impact of heavy metals in the microalga Chlorella sorokiniana and assessment of its potential use in cadmium bioremediation. Aquatic Toxicology239; 105941, 2021 (4.9, D1). <a href=\"https:\/\/doi.org\/10.1016\/j.aquatox.2021.105941\">https:\/\/doi.org\/10.1016\/j.aquatox.2021.105941<\/a><\/p>\n\n\n\n<p><strong>2.<\/strong> Valero Y, Gonz\u00e1lez-Fern\u00e1ndez C, C\u00e1rdenas C, Guzm\u00e1n F, Le\u00f3n R, Cuesta. ANK-lysin peptides ameliorate viral encephalopathy and retinopathy disease and produce partial protection against nodavirus infection. Antiviral Research 2021, 532, 15, 2021. (4.1, Q1). <a href=\"https:\/\/doi.org\/10.1016\/j.aquaculture.2020.736035\">https:\/\/doi.org\/10.1016\/j.aquaculture.2020.736035<\/a><strong>.<\/strong><\/p>\n\n\n\n<p><strong>3.<\/strong>Constantino A; Rodrigues B., Leon R, Barros R, Raposo S.&nbsp; Alternative chemo-enzymatic hydrolysis strategy applied to different microalgae species for bioethanol production. Algal Research. 56, 102329. 2021. (4.0, Q1) <a href=\"https:\/\/doi.org\/10.1016\/j.algal.2021.102329\">https:\/\/doi.org\/10.1016\/j.algal.2021.102329<\/a><\/p>\n\n\n\n<p><strong>4.<\/strong>Cuesta A., Vila M, Molina-Marquez M, Carbajo C, Lebron JA, L\u00f3pez-L\u00f3pez M, L\u00f3pez-Cornejo P, Moy\u00e1 ML, L\u00f3pez-Sanmart\u00edn M, L\u00f3pez JR, Navas JJ., Vigara J, Le\u00f3n R. Influence of adding terminal tags on the structural and antimicrobial properties of the peptide caerin 1.1. Aquaculture. 532, 15, 2021. (3.22, D1) <a href=\"https:\/\/doi.org\/10.1016\/j.aquaculture.2020.736035\">https:\/\/doi.org\/10.1016\/j.aquaculture.2020.736035<\/a>.<\/p>\n\n\n\n<p><strong>5.<\/strong>Le\u00f3n-Vaz A, Romero LC, Gotor C, Le\u00f3n R, Vigara J. &nbsp;Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study. Ecotoxicology and Environmental Safety 207 111301. 2021. (6.291, D1) DOI: <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0147651320311398\">10.1016\/j.ecoenv.2020.111301<\/a>.<\/p>\n\n\n\n<p><strong>6.<\/strong> G\u00f3mez-Villegas, P.; Vigara, J.; Romero, L.; Gotor, C.; Raposo, S.; Gon\u00e7alves, B.; L\u00e9on, R. Biochemical Characterization of the Amylase Activity from the New Haloarchaeal Strain&nbsp;Haloarcula&nbsp;sp. HS Isolated in the Odiel Marshlands.&nbsp;Biology&nbsp;2021,&nbsp;10, 337.(3. 79, Q1) <a href=\"https:\/\/doi.org\/10.3390\/biology10040337\">doi.org\/10.3390\/biology10040337<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2020<\/strong><\/h3>\n\n\n\n<p><strong>1.<\/strong> G\u00f3mez\u2010Villegas P, J. Vigara, M. Vila, J. Varela, L. Barreira. and R. L\u00e9on. Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain). Biology 2020, 9, 298. (3. 79, Q1); doi:<a href=\"https:\/\/www.mdpi.com\/2079-7737\/9\/9\/298\">10.3390\/biology9090298<\/a><\/p>\n\n\n\n<p><strong>2.<\/strong> Le\u00f3n R, Ruiz M, Valero Y, C\u00e1rdenas C, Guzman F, Vila M, Cuesta A. Exploring small cationic peptides of different origin as potential antimicrobial agents in aquaculture. Fish Shellfish Immunol. 2020, 98: 720-727. IF: 3.30, Q1. DOI:<a href=\"https:\/\/doi.org\/10.1016\/j.fsi.2019.11.019\">10.1016\/j.fsi.2019.11.019.<\/a><\/p>\n\n\n\n<p><strong>3.<\/strong> Kaouther Sahli, Mohamed A. Gomri, Julia Esclapez, Patricia Gomez\u2010Villegas, Oulaya Ghennai, Maria\u2010Jose Bonete, Rosa Leon, Karima Kharrou. Bioprospecting and characterization of pigmented halophilic archaeal strains from Algerian hypersaline environments with analysis of carotenoids produced by Halorubrum sp. BS2. J. Basic Microbiology. 2020. DOI: <a href=\"https:\/\/doi.org\/10.1002\/jobm.202000083\">10.1002\/jobm.202000083<\/a><\/p>\n\n\n\n<p><strong>4.<\/strong> Lisa M. Sch\u00fcler, Paulo Duarte, Chlo\u00e9 Placines, Ana Mar\u00eda Molina M\u00e1rquez, Rosa L\u00e9on-Ba\u00f1ares, Katkam N. Gangadhar, Jo\u00e3o Varela and Lu\u00edsa Barreira. Improvement of carotenoid extraction from a recently isolated, robust microalga, Tetraselmis sp. CTP4 (Chlorophyta). Bioprocess and Biosystems Engineering. 2020, 1-12. DOI: <a href=\"https:\/\/doi:10.1007\/s00449-019-02273-9\">https:\/\/doi:10.1007\/s00449-019-02273-9<\/a><\/p>\n\n\n\n<p><strong>5.<\/strong> Molina-M\u00e1rquez a, Vila M, Rengel R, Fern\u00e1ndez E, Garcia-Maroto F, Vigara J, Le\u00f3n R. Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae, 2020, Int. J. Mol. Sci. 21(3), 718. DOI: <a href=\"https:\/\/doi.org\/10.3390\/ijms21030718\">https:\/\/doi.org\/10.3390\/ijms21030718<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2019<\/strong><\/h3>\n\n\n\n<p><strong>1.<\/strong> Le\u00f3n-Vaz A Le\u00f3n R., D\u00edaz-Santos E., Vigara J., Raposo S. Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana. New Biotechnology.51: 31-38 (2019).DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.nbt.2019.02.001\">https:\/\/doi.org\/10.1016\/j.nbt.2019.02.001<\/a><\/p>\n\n\n\n<p><strong>2. <\/strong>Ana Molina-M\u00e1rquez, Marta Vila, Javier Vigara, Ana Borrero and Rosa Le\u00f3n. The Bacterial Phytoene Desaturase-Encoding Gene (CRTI) is an Efficient Selectable Marker for the Genetic Transformation of Eukaryotic Microalgae. Metabolites. 9, 49 (2019). DOI: <a href=\"https:\/\/doi.org\/10.3390\/metabo9030049\">doi:10.3390\/metabo9030049<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2018<\/strong><\/h3>\n\n\n\n<p><strong>1. <\/strong>Patricia G\u00f3mez-Villegas, Javier Vigara and Rosa Le\u00f3n. Characterization of the Microbial Population Inhabiting a Solar Saltern Pond of the Odiel Marshlands (SW Spain). Mar. Drugs. 2018, 16, 332. DOI: <a href=\"https:\/\/doi.org\/10.3390\/md16090332\">https:\/\/doi.org\/10.3390\/md16090332<\/a><\/p>\n\n\n\n<p><strong>2. <\/strong>Rengel R, Smith RT, Haslam R, Sayanova O, Vila M, Le\u00f3n R. Overexpression of acetyl-CoA synthetase (ACS) enhances the biosynthesis of neutral lipids and starch in the green microalga Chlamydomonas reinhardtii. Algal Research. 31: 183-193 (2018).<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2017<\/strong><\/h3>\n\n\n\n<p><strong>1. <\/strong>Sch\u00fcler LM., Peter S.C. Schulze, Hugo Pereira, Lu\u00edsa Barreira, Rosa Le\u00f3n and Jo\u00e3o Varela. Trends and strategies to enhance carotenoid, TAG and LC-PUFA production in microalgae. Algal Research. 25: 263\u2013273 (2017) (3.99, Q1).<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2016<\/strong><\/h3>\n\n\n\n<p><strong>1. <\/strong>Valverde F., Romero-Campereo FJ., Le\u00f3n R., Garc\u00eda-Guerrero M. and Serrano A. New Challenges in microalgal biotechnology Eur. J. protistol. 55:91-105 (2016). (2.58, Q2).<\/p>\n\n\n\n<p><strong>2. <\/strong>D\u00edaz-Santos E., Vila M., Vigara J.,Le\u00f3n R A new approach to express transgenes in microalgae and its use to increase the flocculation ability of Chlamydomonas reinhardtii J. Appl. Phycol. 28:1611-1621 (2016). (2.56, Q1).<\/p>\n\n\n\n<p><strong>3. <\/strong>De la Vega M.; Sayago A., Garc\u00eda-Barneto A.; Ariza J and Le\u00f3n R. Isolation of a new bacterioruberin-producing haloarchaea from the marshlands of the Odiel river in the Southwest Spain Biotechnol. progr. 32(3):592-600 (2016). (2.15, Q1).<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2015<\/strong><\/h3>\n\n\n\n<p><strong>1<\/strong>. Varela, JC; Pereira H, Vila M, Le\u00f3n R. Production of carotenoids by microalgae: achievements and challenges. Photosynth. Res. 125(3):423-36 (2015). (3.5, Q1).<\/p>\n\n\n\n<p><strong>2. <\/strong>D\u00edaz-Santos E, Vila M, de la Vega M, Le\u00f3n R, Vigara J. Study of bioflocculation Induced by Saccharomyces bayanus var. uvarum and flocculating protein factors in microalgae. Algae Research. 8: 23\u201329 (2015). (4.01, Q1).<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2013<\/strong><\/h3>\n\n\n\n<p><strong>1. <\/strong>D\u00edaz-Santos E., Vega M.,Vila M., Vigara J., Le\u00f3n R. Efficiency of different heterologous promoters in the microalga C. reinhardtii. Biotechnol. Prog. 29(2):319-28 (2013).(2.34, Q1).<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2012<\/strong><\/h3>\n\n\n\n<p><strong>1. <\/strong>Vila M., D\u00edaz E., de la Vega M., Rodr\u00edguez H., Vargas A., Le\u00f3n R. Promoter trapping in microalgae by inverse PCR using paromomycin as selective agent. Marine Drugs 10: 2749-2765 (2012). (3.85, Q1).<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2011<\/strong><\/h3>\n\n\n\n<p><strong>1. <\/strong>M de la Vega; E. D\u00edaz, M Vila and R Le\u00f3n. Isolation of a new strain of Picochlorum sp and characterization of its potential biotechnological applications. Biotechnol. Prog. 27(6):1535-43 (2011). (2.34, Q1).<\/p>\n\n\n\n<p><strong>2. <\/strong>Cordero BF, Couso I, Le\u00f3n R, Rodr\u00edguez H, Vargas MA. Enhancement of carotenoids biosynthesis in C. reinhardtii by nuclear transformation using a phytoene synthase gene isolated from C. zofingiensis. Appl. Microb. Biotechnol. 91(2):341-51 (2011). (3.43, Q1).<\/p>\n\n\n\n<p><strong>3. <\/strong>Couso, M Vila , H Rodr\u00edguez, M Vargas and R Le\u00f3n. Overexpression of an exogenous phytoene synthase gene in the unicellular alga Chlamydomonas reinhardtii leads to an increase in the content of carotenoids. Biotechnol. Prog. 27(1):54-60 ( 2011). (2.34, Q1).<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2010<\/strong><\/h3>\n\n\n\n<p><strong>1. <\/strong>BF Cordero, I Obraztsova, L Mart\u00edn, I Couso, R Le\u00f3n, MA Vargas and H Rodr\u00edguez. Isolation and characterization of a lycopene \u03b2-cyclase gene from Chlorella zofingiensis. J. Phycol. 46(6): 1229-1236 (2010). (2.24, Q1).<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><strong>2008<\/strong><\/h3>\n\n\n\n<p><strong>1. <\/strong>M. Vila, I. Couso and R. Le\u00f3n. Carotenoid content in mutants of the chlorophyte Chlamydomonas reinhardtii with low expression levels of phytoene desaturase. Process Biochem. 42:1147-1152 (2008). (2.4, Q1).<\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Publications Genetic Engineering Of Photosynthetic Organisms Unit Laboratory Menu INTERNATIONAL PUBLICATIONS 2026 Molina\u2010M\u00e1rquez, A, S. Kelterborn, P. Hegemann, M. P\u00e9rezRodr\u00edguez, J. Vigara and R. Le\u00f3n. 2026. Characterization of Phytoene Desaturase Knockout Carotenoid\u2010Deficient Microalgal Mutants Generated by Cas9\u2010Ribonucleoprotein Complexes. Physiologia Plantarum e70811.\u00a0https:\/\/doi.org\/10.1111\/ppl.70811 2025 \u00c1ngeles Mor\u00f3n-Ortiz, Mar Ferrando-Marco, Antonio Le\u00f3n-Vaz, Rosa Le\u00f3n, Paula Mapelli-Brahm, Michalis Barkoulas, Mel\u00e9ndez &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"\" href=\"https:\/\/www.uhu.es\/rensma\/en\/publications-mgof\/\"> <span class=\"screen-reader-text\">Publications Genetic Engineering Of Photosynthetic Organisms Unit<\/span> Leer m\u00e1s &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"","footnotes":""},"_links":{"self":[{"href":"https:\/\/www.uhu.es\/rensma\/wp-json\/wp\/v2\/pages\/4926"}],"collection":[{"href":"https:\/\/www.uhu.es\/rensma\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.uhu.es\/rensma\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.uhu.es\/rensma\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.uhu.es\/rensma\/wp-json\/wp\/v2\/comments?post=4926"}],"version-history":[{"count":11,"href":"https:\/\/www.uhu.es\/rensma\/wp-json\/wp\/v2\/pages\/4926\/revisions"}],"predecessor-version":[{"id":6256,"href":"https:\/\/www.uhu.es\/rensma\/wp-json\/wp\/v2\/pages\/4926\/revisions\/6256"}],"wp:attachment":[{"href":"https:\/\/www.uhu.es\/rensma\/wp-json\/wp\/v2\/media?parent=4926"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}