Seville is the main city in the vicinity of Matalascañas and one of the most important Spanish ones (the fourth by population). Seville can be easily reached
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Huelva can be also reached from the international airport of Faro (Portugal) by regular buses. In this link you can check all the airlines flying to Faro (there are several low-cost ones). A transfer service from the airport to Matalascañas is also available by 30 euros.