Facilities

ENVIRONMENTAL AND BIOANALYTICAL CHEMISTRY UNIT (FQM-141)

Laboratory Menu

LABORATORIES

The Environmental and Bioanalytical Chemistry Group is located at the Faculty of Experimental Sciences (University of Huelva):

  • Laboratory for simple preparison (Ex-P1-N5-01)- 40 m2
  • Laboratory for Metabolomics (Ex-P1-N5-08)- 60 m2
  • Laboratory for Trace Metal Analysis (Ex-P1-N5-07)- 60 m2
  • Laboratory for Chemical Spaciation and Metallomics (Ex-P2-N5-07)- 60 m2

INSTRUMENTATION

Chromatography and Electrophoresis

  • Gas chromatograph coupled to ion trap mass spectrometer TRACE GC ULTRA ITQ 900 (Thermo Fisher Scientific)
  • Gas chromatograph coupled to electron capture detector (GC-µECD, model 6890, Agilent Technologies)
  • Liquid chromatograph with UV-vis spectrophotometric detector (HPLC-UV, Thermo Fisher Scientific, Accela PDA 80 Hz Detector)
  • Liquid chromatograph with molecular fluorescence detector (HPLC-FLD, Agilent Technologies)
  • Nano and micro-HPLC (Agilent Technologies)
  • Gas chromatograph coupled to flame ionization detector (GC-FID) (Varian)
  • Gas chromatograph coupled to pulsed flame photometric detector (GC-PFPD) (Varian)
  • Capillary electrophoresis with UV detector (Beckman)
  • Bidimensional electrophoresis system.

Atomic mass spectrometry

  • Inductively coupled plasma coupled to cuadrupole reaction cell (ICP-MS) Thermo Xseries 2 (con celda de colisión/reacción). Sistema de introducción automática de muestras
  • Inductively coupled plasma with triple quadrupole (ICP-QQQ-MS) Agilent 8800 (Agilent Technologies). Autosampler and bio-inert HPLC model 1260 Infinity.
  • Laser ablation system NW100181

Organic mass spectrometry

  • Ultra-high performance liquid chromatography coupled to cuadrupole time of flight mass spectrometer (UHPLC-QTOF) Agilent 6550 iFunnel (Agilent Technologies)
  • Ultra-high performance liquid chromatography model 1290 Infinity II and ion mobility (Agilent GS 6560, Agilent Technologies) coupled to cuadrupole time of flight mass spectrometer.
  • Matrix assisted laser desorption ionization coupled to time of flight mass spectrometer (MALDI-TOF-MS) Voyager Applied Biosystem

Sample preparation

  • Pressurized liquid extraction
  • Focalized microwave
  • Microwave system for simple mineralization with low and high-volume reactors (CEM Mars 240/50)
  • Crio-homogeneizers for tissues.
  • Tissue Lyser (Fisher Scientific 1.0 DISRUPTOR GENIE 230 v)
  • Ultrasound bath
  • Ultrasound probe (SONOPLUS mini20)

Other instrumentation and material

  • Termostatized UV-vis spectrophotometer with peltier system (Fisher Scientific, Evolution 201)
  • 2 centrifuges, one of them termostatized (Eppendorf AG Centrifuge 5804 R
  • Speed vaccum (SpeedVac SPD 111V)
  • Rotary evaporators
  • Filtration systems
  • Ultrapure water purification system (Milli-Q Direct 8)
  • 2 Stoves
  • 4 Fridges
  • 2 Freezers -20 º C
  • 3 Freezers a -80ºC

PROJECTS FOR FACILITIES ACQUISITION

  • Instrumental platform for lipidomics, proteomics and metallomics.
  • Instrumental platform for metabolomics.
  • Instrumental platform for metallomics, proteomics and environmental pollutants related to health, environment and food analysis based on UHPLC-ICP-QQQ-MS.
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